Neurons derived from human embryonic stem cells extend long-distance axonal projections through growth along host white matter tracts after intra-cerebral transplantation

M Denham; CL Parish; B Leaw; J Wright; CA Reid; S Petrou; M Dottori; LH Thompson

Journal article

Neurons derived from human embryonic stem cells extend long-distance axonal projections through growth along host white matter tracts after intra-cerebral transplantation

M Denham, CL Parish, B Leaw, J Wright, CA Reid, S Petrou, M Dottori, LH Thompson

Frontiers in Cellular Neuroscience | FRONTIERS MEDIA SA | Published : 2012

DOI: 10.3389/fncel.2012.00011

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Abstract

Human pluripotent stem cells have the capacity for directed differentiation into a wide variety of neuronal subtypes that may be useful for brain repair. While a substantial body of research has lead to a detailed understanding of the ability of neurons in fetal tissue grafts to structurally and functionally integrate after intra-cerebral transplantation, we are only just beginning to understand the in vivo properties of neurons derived from human pluripotent stem cells. Here we have utilised the human embryonic stem (ES) cell line Envy, which constitutively expresses green fluorescent protein (GFP), in order to study the in vivo properties of neurons derived from human ES cells. Rapid and e..

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University of Melbourne Researchers

Clare Parish Author

Christopher Reid Author

Lachlan Thompson Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We are grateful for expert technical assistance provided by Ms. Doris Thomas, Ms. MongTien, Dr. Brock Conley, and Ms. Jesse Leung. This project was supported by funding from the Australian National Health and Medical Research Council (project grants 508992 and 628542), the Motor Neuron Disease Research Institute (grant-in-aid 2011), the Friedreich's Ataxia Foundation, and the Victorian Government through the Operational Infrastructure Scheme. Lachlan H. Thompson is supported by an NH&MRC Career Development Fellowship, Clare L. Parish receives a Senior Medical Research Fellowship through the Viertel Foundation, and Mark Denham is supported by an NH&MRC Peter Doherty Fellowship (520165). Antibodies obtained from the Developmental Studies Hybridoma Bank (DSHB) were developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.